中国组织工程研究

中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (16): 2851-2858.doi: 10.3969/j.issn.2095-4344.2013.16.001

• 组织工程口腔材料 tissue-engineered oral materials •    下一篇

小型猪自体牙周膜干细胞与复合材料修复牙周骨缺损

杨斯惠1，钟良军2，张鹏涛3，张 远3，张源明4，马路平1，徐 艳5   

  1. 1新疆医科大学第一附属医院口腔科，新疆维吾尔自治区乌鲁木齐市 830054
    2杭州师范大学临床医学院，浙江省杭州市 310012
    3杭州师范大学附属医院口腔科，浙江省杭州市 310015
    4新疆医科大学第一附属医院心脏中心，新疆维吾尔自治区乌鲁木齐市 830054
    5南京医科大学附属口腔医院，江苏省南京市 210000
  • 收稿日期:2012-12-07 修回日期:2013-02-27 出版日期:2013-04-16 发布日期:2013-04-16
  • 通讯作者: 张源明，博士，教授，博士生导师，新疆医科大学第一附属医院心脏中心，新疆维吾尔自治区乌鲁木齐市 830054 zymdxx@163.com
  • 作者简介:杨斯惠★，女，1987年生，辽宁省新民市人，汉族，新疆医科大学在读硕士，主要从事牙周病防治的研究。yangsihuishuoer@sina.com
  • 基金资助:

    自治区科技支疆项目(201091144)；浙江省教育厅科研项目(Z201122835)。

Autologous periodontal ligament stem cells combined with composites repair periodontal bone defects in miniature pigs

Yang Si-hui1, Zhong Liang-jun2, Zhang Peng-tao3, Zhang Yuan3, Zhang Yuan-ming4, Ma Lu-ping1,Xu Yan5   

  1. 1 Department of Stomatology, the First Affiliated Hospital of Xinjiang Medical University, Urumqi
    830054, Xinjiang Uygur Autonomous Region, China
    2 Clinical College of Hangzhou Normal University, Hangzhou 310015, Zhejiang Province, China
    3 Department of Stomatology, Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, Zhejiang Province, China
    4 Department of Cardiology, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054,
    Xinjiang Uygur Autonomous Region, China
    5 Stomatological Hospital of Nanjing Medical University, Nanjing 210000, Jiangsu Province, China
  • Received:2012-12-07 Revised:2013-02-27 Online:2013-04-16 Published:2013-04-16
  • Contact: Zhang Yuan-ming, Doctor, Professor,Doctoral supervisor,Department of Cardiology, the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054, Xinjiang Uygur Autonomous Region, China zymdxx@163.com
  • About author:Yang Si-hui★, Studying for master’s degree, Department of Stomatology, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China yangsihuishuoer@sina.com
  • Supported by:

    Regional Scientific and Technological Supporting Xinjiang Project, No.201091144*; Scientific Research Projects of Zhejiang Provincial Education Department, No. Z201122835*

PDF

793

被引次数

10

摘要：

背景：研究发现应用细胞与支架材料相结合可达到较理想的牙周骨缺损修复效果。
目的：观察小型猪自体牙周膜干细胞联合羟基磷灰石生物陶瓷复合物修复牙周二类骨缺损的效果。
方法：共纳入6只贵州小型猪，用台式慢机磨除的方法建立贵州小型猪上下颌骨二类牙周骨缺损模型，骨缺损位于第三前磨牙和第四前磨牙之间，暴露第四前磨牙的近中牙根。实验组应用自体上下颌尖牙牙周膜干细胞复合羟基磷灰石生物陶瓷进行缺损修复；对照组应用单纯羟基磷灰石生物陶瓷进行修复；以未进行任何修复的作为模型组。各组在骨缺损区域表面覆盖口腔生物膜。造模后12周，取各组缺损牙周组织，通过临床观察，头颅螺旋CT平扫及三维重建和苏木精-伊红染色观察牙周骨组织成骨愈合情况。
结果与结论：临床观察显示实验组牙周缺损愈合最好。头颅螺旋CT见实验组缺损区骨密度与周围正常牙槽骨密度无明显差异，对照组及模型组缺损区缺损仍清晰可见，呈不完全愈合状态。苏木精-伊红染色见实验组新生牙槽骨已充满骨缺损处，建立了正常的钙化骨结构；对照组新生牙槽骨尚未充满骨缺损处，钙化程度极低；模型组可见大量的胶原纤维，缺损区仍大量空缺，未见明显新骨形成。可见牙周膜干细胞联合引导组织再生能有效提高小型猪牙周二类骨缺损愈合和牙周再生能力，修复牙周骨缺损。

关键词: 生物材料, 组织工程口腔材料, 干细胞移植, 牙周膜干细胞, 引导组织再生, 成骨, 牙周再生, 骨缺损, 牙周病, 省级基金

Abstract:

BACKGROUND: Studies have proved that autologous periodontal ligament stem cells combined with scaffold materials can achieve better effect on the repair of periodontal bone defects.
OBJECTIVE: To observe the effect of miniature pig autologous periodontal ligament stem cells combined with hydroxyapatite bioceramic composites in the repair of category Ⅱ periodontal bone defects.
METHODS: Six Guizhou miniature pigs were included, and were used to establish the miniature pig models of upper and lower jaw category Ⅱ periodontal bone defects. The bone defects were located between the third premolar and fourth premolar, and the near root of fourth premolar was exposed. The defects in the experimental group were repaired with periodontal ligament stem cells obtained from the autologous maxillary and mandibular canine and combined with hydroxyapatite bioceramic; the defects in the control group were repaired with hydroxyapatite bioceramic simplely; and the model group did not repaired. The defects in each group were covered with oral biofilm. At 12 weeks after modeling, the defect periodontal tissues were obtained from each group, and then the osteogenesis healing of the periodontal bone tissue was observed through clinical observation, heal spiral CT scanning, three-dimensional reconstruction and hematoxylin-eosin staining.
RESULTS AND CONCLUSION: Clinical observation showed that healing of periodontal defects in the experimental group was the best. Head spiral CT scanning showed that there was no significant difference of bone mineral density between bone defect area and surrounding normal alveolar bone, and the defects in the control group and the model group were still clear visible in the incomplete healing state. Hematoxylineosin staining showed that the defect area in the experimental group was filled with newborn alveolar bone completely, and the normal calcified bone structure was established; in the control group, the defect area was filled with newborn alveolar bone incompletely with low calcification degree; a large number of collagen fibers could be seen in the model group, and the defect area was not filled and no new bone formed. It is visible that periodontal ligament stem cells combined with guided tissue regeneration can effectively promote healing of category II periodontal bone defects and periodontal regeneration in miniature pigs, thus repairing periodontal bone defects.

Key words: biomaterials, tissue-engineered oral materials, stem cell transplantation, periodontal ligament stem cells, guided tissue regeneration, osteogenesis, periodontal regeneration, bone defect, periodontal disease, provincial grants-supported paper

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